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phosphatidylcholine  (Avanti Polar)
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Avanti Polar phosphatidylcholine
Phosphatidylcholine, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polar lipid extract ple  (Avanti Polar)
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Avanti Polar polar lipid extract ple
Polar Lipid Extract Ple, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total e coli lipids  (Avanti Polar)
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Avanti Polar total e coli lipids
Total E Coli Lipids, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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porcine brain polar lipid extract  (Avanti Polar)
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Avanti Polar porcine brain polar lipid extract
Porcine Brain Polar Lipid Extract, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli  (Croda International Plc)
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Croda International Plc escherichia coli
Escherichia Coli, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l α phosphatidylcholine  (Avanti Polar)
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Avanti Polar l α phosphatidylcholine
L α Phosphatidylcholine, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain total lipid extract  (Avanti Polar)
96
Avanti Polar brain total lipid extract
Analysis of the bovine <t>brain</t> <t>lipid</t> <t>extract</t> in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain <t>total</t> lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.
Brain Total Lipid Extract, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain total lipid extract - by Bioz Stars, 2026-03
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egg l α phosphatidylcholine pcs100  (Avanti Polar)
96
Avanti Polar egg l α phosphatidylcholine pcs100
Analysis of the bovine <t>brain</t> <t>lipid</t> <t>extract</t> in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain <t>total</t> lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.
Egg L α Phosphatidylcholine Pcs100, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli polar lipid extract  (Avanti Polar)
95
Avanti Polar e coli polar lipid extract
Analysis of the bovine <t>brain</t> <t>lipid</t> <t>extract</t> in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain <t>total</t> lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.
E Coli Polar Lipid Extract, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain ceramide  (Avanti Polar)
98
Avanti Polar brain ceramide
Analysis of the bovine <t>brain</t> <t>lipid</t> <t>extract</t> in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain <t>total</t> lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.
Brain Ceramide, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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soy phosphatidylcholine pc  (Avanti Polar)
97
Avanti Polar soy phosphatidylcholine pc
Analysis of the bovine <t>brain</t> <t>lipid</t> <t>extract</t> in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain <t>total</t> lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.
Soy Phosphatidylcholine Pc, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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porcine brain sphingomyelin lipids  (Avanti Polar)
98
Avanti Polar porcine brain sphingomyelin lipids
Analysis of the bovine <t>brain</t> <t>lipid</t> <t>extract</t> in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain <t>total</t> lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.
Porcine Brain Sphingomyelin Lipids, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Analysis of the bovine brain lipid extract in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain total lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.

Journal: Journal of Lipid Research

Article Title: Direct analysis of sialylated or sulfated glycosphingolipids and other polar and neutral lipids using TLC-MS interfaces [S]

doi: 10.1194/jlr.D046128

Figure Lengend Snippet: Analysis of the bovine brain lipid extract in the negative ion mode of LESA and CAMAG TLC-MS interfaces coupled with ESI-MS/MS. TLC plates stained with orcinol (A) and primuline (C). Lane 1, monosialoganglioside mixture (GM1, GM2, and GM3); lane 2, disialoganglioside mixture (GD1a, GD1b, and GD3); lane 3, GT1b and GQ1b; lane 5, ST mixture (d18:1/C12:0 ST, d18:1/C24:1 ST, d18:1/C24:0 ST, and d18:1/C12:0 di-ST); and lanes 4 and 6, bovine brain total lipid extracts. B: The band containing each of the GD1 isomers was desorbed from the TLC plate by LESA; the [M – H]− peaks of the two homologs were isolated in Q1 and subjected to CID MS/MS. *The signature fragments that differentiate the glycan structures of GD1a and GD1b.

Article Snippet: The acidic GSL standards, GM1 and ST (d18:1/C24:1), and the bovine brain total lipid extract were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL).

Techniques: Tandem Mass Spectroscopy, Staining, Isolation